Detection of blaSPM-1 and blaSIM-2 Metallo-β-Lactamases Genes in Imipenem-Resistant Pseudomonas aeruginosa Clinical Isolates Recovered from Two University Hospitals in Egypt

Emara, Mohamed; El-Domany, Ramadan; Abu El-Magd, Mohamed; Moustafa, Walaa; Alkemary, Nesma

doi:10.21608/aprh.2020.29194.1106

Detection of blaSPM-1 and blaSIM-2 Metallo-β-Lactamases Genes in Imipenem-Resistant Pseudomonas aeruginosa Clinical Isolates Recovered from Two University Hospitals in Egypt

Document Type : Research Article

Authors

¹ Department of Microbiology and Immunology, Faculty of Pharmacy, Helwan University, Egypt

² Faculty of Pharmacy and Pharmaceutical Industries - KafrElsheikh University, Egypt

³ Department of Anatomy, Faculty of Veterinary Medicine, Kaferelsheik University, Egypt

⁴ Department of Microbiology and Immunology, Faulty of Pharmacy, Kafr- Elsheikh University, Egypt

10.21608/aprh.2020.29194.1106

Abstract

Objectives: Unraveling mechanisms whereby Pseudomonas aeruginosa becomes resistant to carbapenems through testing 114 non-duplicate P. aeruginosa clinical isolates for their susceptibility to various classes of antibiotics and scrutinizing the production of metallo-β-lactamases (MBLs) by tested isolates. Methods: Susceptibility testing of P. aeruginosa to different antibiotics was determined by Kirby-Bauer disk diffusion method and MBLs production by tested isolates was studied phenotypically and genotypically and PCR products were confirmed by sequencing Results: All tested clinical isolates showed eminent resistance to the majority of tested antibiotics and 14 isolates were imipenem (IPM)-resistant. Furthermore, IPM-resistant isolates were verified to be MBLs-producers. MBLs-encoding genes bla_SIMand bla_SPM genes were detected by PCR where four isolates were found to harbor bla_SPM gene while only one isolate harbored bla_SIM gene. The correct size of PCR products of bla_SIM and bla_SPM genes were sequenced and sequences were submitted to the GenBank databases and assigned the accession numbers KX452682 and KX452683 for bla_SIM-2and bla_SPM-1, respectively. Conclusion: Here, we report the emanation of P. aeruginosa clinical isolates harboring bla_SPM-1 and bla_SIM-2genes. This may reflect the substantial increase in the rate of imipenem resistance due to MBL in P. aeruginosa clinical isolates from Egypt. Early detection and infection-control practices are of the best antimicrobial strategy for combating this organism.

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