Mohammed, N., Kamal, A., Abdelhady, M., Haggag, E. (2021). HPLC Phenolic Profiling of Alcoholic Extracts of Three Abutilon Species. Journal of Advanced Pharmacy Research, 5(4), 371-376. doi: 10.21608/aprh.2021.92719.1139
Nermine M. Mohammed; Amel M. Kamal; Mohamed I. S. Abdelhady; Eman G. Haggag. "HPLC Phenolic Profiling of Alcoholic Extracts of Three Abutilon Species". Journal of Advanced Pharmacy Research, 5, 4, 2021, 371-376. doi: 10.21608/aprh.2021.92719.1139
Mohammed, N., Kamal, A., Abdelhady, M., Haggag, E. (2021). 'HPLC Phenolic Profiling of Alcoholic Extracts of Three Abutilon Species', Journal of Advanced Pharmacy Research, 5(4), pp. 371-376. doi: 10.21608/aprh.2021.92719.1139
Mohammed, N., Kamal, A., Abdelhady, M., Haggag, E. HPLC Phenolic Profiling of Alcoholic Extracts of Three Abutilon Species. Journal of Advanced Pharmacy Research, 2021; 5(4): 371-376. doi: 10.21608/aprh.2021.92719.1139
HPLC Phenolic Profiling of Alcoholic Extracts of Three Abutilon Species
Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, 11795, Egypt.
Abstract
Objectives: This study was aimed to analyzing the phenolic constituents of the 80% methanol extracts of three Abutilon species (A. pannosum, A. hybridum and A. hirtum) (family Malvaceae). Methods: Defatted 80% methanol extracts of A. pannosum, A. hybridum and A. hirtum aerial parts (leaves and stems) were, each, analyzed using high performance liquid chromatographic (HPLC) technique against external standard calibration at λ 280 and 330 nm. Results: Twelve phenolic acids were tentatively identified from A. pannosum, A. hybridum and A. hirtum representing 9.03, 12.23 and 20.51%, respectively. The major identified phenolic acids peaks were that of protocatechuic acid in A. pannosum (1.75%), ferulic acid in A. hybridum (2.09%) and ellagic acid in A. hirtum (4.0%). On the other hand, 21 flavonoids were tentatively identified from A. pannosum representing (53.78%), in comparison with 20 identifiedfrom A. hybridum (42.12%) and 19 identified from A. hirtum (35.68%). Kaempferol 3-O- [2\\-p < /em>-coumaroyl-β-D-glucopyranoside] was the major identified flavonoid glycoside inA. pannosum and A. hybridum methanolic extracts (13.27 and 5.13%, respectively)while quercitrin was the major flavonoid glycoside identified inA. hirtum (3.21%).The major flavonoid aglycones identified were luteolin (3.68%) inA. pannosum, apigenin (9.46%) in A.hybridumand quercetin (5.85%) in A. hirtum. Conclusion: The present study revealed that the investigated extracts are rich in phenolic contents; A. pannosum has the highest concentration of flavonoids and the least concentration of phenolic acids, where A. hirtum has the highest concentration of phenolic acids, showing promising phenolic content for further study of their isolation, identification as well as evaluation of their biological activity.
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