Simultaneous Determination of Amlodipine and Hydrochlorothiazide in Pharmaceutical Preparations by Differential Pulse Voltammetry Method

Yilmaz, Bilal; Kocak, Umit

doi:10.21608/aprh.2018.5851.1068

Simultaneous Determination of Amlodipine and Hydrochlorothiazide in Pharmaceutical Preparations by Differential Pulse Voltammetry Method

Document Type : Research Article

Authors

Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey

10.21608/aprh.2018.5851.1068

Abstract

Objectives: In this study, a new, fast and reliable differential pulse voltammetry (DPV) method was developed and validated for the simultaneous determination of amlodipine (AML) and hydrochlorothiazide(HCT) in pharmaceutical preparations. Methods: Electrochemical behavior and simultaneous voltammetric determination of AML and HCT
were investigated using glassy carbon electrode. Validation parameters such as specificity, linearity, accuracy, precision, ruggedness, stability, limit of quantification and limit of detection were studied according to the International
Conference on Harmonisation (ICH) Guidelines. Results: The linearity of this developed method was established in the concentration range of 2.5-30 μg/mL for AML and HCT, respectively. The precision was less than 5.34 and 2.91 %, determined from quality control samples for AML and HCT, and accuracy was within 2.57 and 3.60 % in terms of relative error, respectively. The percentage recovery obtained for AML and HCT in pharmaceutical preparations were
99.6 and 100.1 %, respectively. Limits of detection and quantification for AML and HCT were 0.80 and 2.40 µg/mL, respectively. Conclusion: The developed DPV method can be used for routine analysis of AML and HCT in pharmaceutical preparations.

Highlights

We have been developed a new method for the simultaneous determination of amlodipine (AML) and hydrochlorothiazide(HCT) in pharmaceutical preparations.
The method was fully validated according to ICH Guidelines.
No other literature was observed differential pulsevoltammetry method for the simultaneous determination of AML and HCT in pharmaceutical preparations.
The method should be applied for the direct simultaneous determination of AML and HCT in pharmaceutical preparations without any extraction or evaporation steps prior to drug assay.

Keywords