Khazaal, H., Bassyoni, R., Bishr, M., El-Sayed, E., El Dib, R., Soliman, H. (2023). Polyphenolic Profiling and Analgesic Activity of Citrullus lanatus var. citroides Fruit. Journal of Advanced Pharmacy Research, 7(3), 127-132. doi: 10.21608/aprh.2023.217966.1221
Heba Khazaal; Reham Bassyoni; Mokhtar Bishr; Elsayed El-Sayed; Rabab El Dib; Hesham Soliman. "Polyphenolic Profiling and Analgesic Activity of Citrullus lanatus var. citroides Fruit". Journal of Advanced Pharmacy Research, 7, 3, 2023, 127-132. doi: 10.21608/aprh.2023.217966.1221
Khazaal, H., Bassyoni, R., Bishr, M., El-Sayed, E., El Dib, R., Soliman, H. (2023). 'Polyphenolic Profiling and Analgesic Activity of Citrullus lanatus var. citroides Fruit', Journal of Advanced Pharmacy Research, 7(3), pp. 127-132. doi: 10.21608/aprh.2023.217966.1221
Khazaal, H., Bassyoni, R., Bishr, M., El-Sayed, E., El Dib, R., Soliman, H. Polyphenolic Profiling and Analgesic Activity of Citrullus lanatus var. citroides Fruit. Journal of Advanced Pharmacy Research, 2023; 7(3): 127-132. doi: 10.21608/aprh.2023.217966.1221
Polyphenolic Profiling and Analgesic Activity of Citrullus lanatus var. citroides Fruit
1Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Ain-Helwan, Cairo 11795, Egypt.
2Plant General Manager and Technical Director of the Arab Company for Pharmaceuticals and Medicinal Plants, Cairo, Egypt.
3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Helwan University, Ain-Helwan, Cairo 11795, Egypt.
4Egypt Japan University for Science and Technology (EJUST), PharmD program. P. O. Box 179 New Borg El-Arab City, Alexandria, Egypt.
Abstract
Objectives: This study was designed for preliminary phytochemical screening of Citrulluslanatus var. citroides fruit pulp secondary metabolites. In addition, the polyphenolic compounds of the methanolic extract (ME) and its n-butanol soluble fraction (n-BF) were analyzed, and the in vivo analgesic activity of the richest polyphenolic fraction was evaluated. Methods: TheMEwas phytochemically screened for various secondary metabolites. Moreover, ME and n-BF polyphenolic profiling was performed using high performance liquid chromatography (HPLC) against external standards. The analgesic activity of the polyphenolic rich fraction (n-BF) was evaluated in vivo using acetic acid-induced abdominal writhing and hot plate tests. Results: Preliminary phytochemical screening revealed the presence of carbohydrate and /or glycosides, flavonoids, saponins, tannins, and unsaturated sterols and /or triterpenes, while alkaloids and/or compounds containing nitrogenous bases, anthraquinones, cardiac glycosides, and coumarins were absent. HPLC polyphenolic profiling showed the tentative identification of 11 phenolic compounds in ME (19.6959%), including 6 phenolic acids and 5 flavonoids, with gallic acid and rutin being the major identified ones; respectively. As well as, 17 phenolic compounds were tentatively identified in the n-BF (75.0771%), including 10 phenolic acids with chlorogenic acid as the major identified one, and 7 flavonoids with naringenin as the major one.Then-BF showed significant peripheral and central analgesic activity in a dose-dependent manner (p < 0.05), with n-BF at a dose of 1000 mg/kg being the most potent. Conclusion: The present study revealed that the n-BF was highly rich in phenolic compounds. These phenolic compounds were reported for the first time in C. lanatus var. citroides. The n-BF showed significant analgesic activity which was evaluated for the first time for the plant.
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