Validated Spectrofluorimetric Method for the Determination of Cefoxitin Sodium in Its Pure Form and Powder for Injection via Derivatization with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Attia, Khalid; Magdy, Nancy; Mohamed, Ghada

doi:10.21608/aprh.2017.4042

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	Validated Spectrofluorimetric Method for the Determination of Cefoxitin Sodium in Its Pure Form and Powder for Injection via Derivatization with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)
Article 5, Volume 1, Issue 4, October 2017, Page 209-2015 PDF (626.2 K)
DOI: 10.21608/aprh.2017.4042
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Authors
Khalid Attia¹; Nancy Magdy²; Ghada Mohamed^* ¹
¹Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
²Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Abstract
Objectives: An accurate and precise spectrofluorimetric methodwas developed and validated for the determination of β-lactam antibiotic named; cefoxitin sodiumin its pure form and powder for injection. Methods: Based on nucleophilic substitution reaction of target drug with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) to form a highly fluorescent fluorophore measured at 540 nm after excitation at 460 nm. Results: Under optimum condition, the proposed method obeys Beer’s law in range (0.5-7 µg mL^-1) and the reaction mechanisms were presented. Conclusion: The method was validated according to ICH guideline for accuracy, precision and was successfully applied for the determination of the drug in its pure form and powder for injection. The obtained results were statistically compared with those of the reported method and found to be in good agreement.
Keywords
Cefoxitin sodium; Spectrofluorimetric method; 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole; NBD-Cl


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