Antimicrobial and Antioxidant Potential of Optimized Copper Oxide Nanoparticles of Cassia angustifolia Leaf Extract against Resistant Isolates

Sharma, Shikha; Jangid, Anil Kumar

doi:10.21608/aprh.2025.407576.1331

Antimicrobial and Antioxidant Potential of Optimized Copper Oxide Nanoparticles of Cassia angustifolia Leaf Extract against Resistant Isolates

Document Type : Research Article

Authors

Department of Pharmaceutical Science, Lords University, Alwar, Rajasthan India 301001

10.21608/aprh.2025.407576.1331

Abstract

Objectives: The current study was aimed at synthesizing copper oxide nanoparticles (CuO-NPs) using the leaf extract of Cassia angustifolia using a green, eco-friendly procedure and establishing the antimicrobial and antioxidant capacity of these nanoparticles against drug-resistant microbial isolates. It was also the purpose of the study to compare the biological activity of CuO-NPs and plain ethanol extract of Cassia angustifolia. Methods: The green synthesis technique was used to prepare CuO-NPs in which the reducing and stabilizing agent was Cassia angustifolia leaf extract. The prepared nanoparticles were optimized and characterized (this fact is skipped to make the abstract short). Their activities were also measured in terms of their antioxidant level by DPPH radical scavenging activity and total antioxidant capacity. The antibacterial activity was established by the procedure of well diffusion agar against Gram-positive (Bacillus subtilis, Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae) bacteria strains. Results: The optimized CuO-NPs manifested the drastic improvement in antioxidant activity with the inhibition of DPPH-free radical formation (85.28%) compared to plain ethanol extract (62.12%). It was also discovered that dose-dependent reduction in total antioxidant activity occurred. The CuO-NPs had great antibacterial activities, and zones of inhibition were between 18.24 and 25.78 mm against a variety of strains assayed. The modified CuO-NPs were very sensitive, with an average of 60-80 percent of the isolates tested. Conclusion: The green method used to synthesize CuO-NPs using an extract of Cassia angustifolia leaves showed a high level of antibacterial and antioxidant activity. These outcomes are suggestive of the fact that they can be used in medical fields as alternative treatment methods, especially to overcome drug-resistant infections. This enhanced activity is explainable by both the presence of compounds of bioactive secondary metabolites inside it, the extract itself, and the influence of the polarity of solvents used in synthesis.

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