Gabr, N., Mina, S., Talat, J., Fahmy, S. (2020). Determination of Volatile Constituents of Cucumis melo L. var. aegyptiacus cv. Ismailawi Fruits and Evaluation of Its Cytotoxic Activity. Journal of Advanced Pharmacy Research, 4(1), 7-12. doi: 10.21608/aprh.2019.18355.1091
Nagwan Gabr; Suzan Mina; Jaky Talat; Sherifa Fahmy. "Determination of Volatile Constituents of Cucumis melo L. var. aegyptiacus cv. Ismailawi Fruits and Evaluation of Its Cytotoxic Activity". Journal of Advanced Pharmacy Research, 4, 1, 2020, 7-12. doi: 10.21608/aprh.2019.18355.1091
Gabr, N., Mina, S., Talat, J., Fahmy, S. (2020). 'Determination of Volatile Constituents of Cucumis melo L. var. aegyptiacus cv. Ismailawi Fruits and Evaluation of Its Cytotoxic Activity', Journal of Advanced Pharmacy Research, 4(1), pp. 7-12. doi: 10.21608/aprh.2019.18355.1091
Gabr, N., Mina, S., Talat, J., Fahmy, S. Determination of Volatile Constituents of Cucumis melo L. var. aegyptiacus cv. Ismailawi Fruits and Evaluation of Its Cytotoxic Activity. Journal of Advanced Pharmacy Research, 2020; 4(1): 7-12. doi: 10.21608/aprh.2019.18355.1091
Determination of Volatile Constituents of Cucumis melo L. var. aegyptiacus cv. Ismailawi Fruits and Evaluation of Its Cytotoxic Activity
1Pharmacognosy Department, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
2Pharmacognosy Department, Faculty of Pharmacy, 6th October University
3Department of Pharmacognosy, Faculty of Pharmacy, 6th October University
Abstract
Objectives:Cucumis melo L. var. aegyptiacus cv. Ismailawi plant is a member of family Cucurbitaceae. This study aimed to identify volatile components of the pulp of the fruit and also to evaluate its cytotoxic activity. Methods: Automated rapid headspace solid phase micro extraction (SPME) was applied for extraction of the volatile components combined with gas chromatography coupled to mass spectrometry GC/MS for identification of the compounds responsible for the specific aroma of the fruits, MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay) was used for the evaluation of the cytotoxic activity of both petroleum ether and defatted methanol extracts of the fruits’ pulp against 3 different tumor cell lines; colon (Caco-2), hepatic (HepG2) and cervix (HeLa) carcinoma cells. Results: Twenty volatile components were identified in the pulp of the fruits which were dominated by esters as the major class of components, with hexyl acetate representing the major constituent (23.07%). Petroleum ether extract of the pulp displayed mild cytotoxic activity against HepG2 cell line with IC50 of (31.6) µg/ml, all results were standardized against doxorubicinpositive control. Conclusion: This is the first report for the evaluation of the volatile constituents of Cucumis melo L. var. aegyptiacus cv. Ismailawi fruits which can serve as a base for its differentiation from other similar varieties in the same family.
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