Determination of Flucloxacillin-sodium in Binary Mixture with Ampicillin-trihydrate Using Univariate and Multivariate Spectrophotometric Methods: A Comparative Study

Attia, Khalid Abdel-Salam; Abdel-Aziz, Omar; Nasef, Nancy; Mohamed, Ghada

doi:10.21608/aprh.2018.2362.1047

Determination of Flucloxacillin-sodium in Binary Mixture with Ampicillin-trihydrate Using Univariate and Multivariate Spectrophotometric Methods: A Comparative Study

Document Type : Research Article

Authors

¹ Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.

² Pharmaceutical Analytical Chemistry, Faculty of pharmacy, Ain shams Univeristy

10.21608/aprh.2018.2362.1047

Abstract

Objectives: The aim of this study was to develop four simple and accurate spectrophotometric methods for determination of flucloxacillin-sodium in binary mixture with ampicillin-trihydrate without separation. Methods: One of them was a univariate constant center method and the other three methods were multivariate chemometric methods named; Savitsky-Golay filters, continuous wavelet transform of ratio spectra and wavelet transform of first derivative of the ratio spectra. Results: The proposed methods adopted for selective determination of flucloxacillin-sodium and obey Beer’s law in the range (2-20 µg mL^-1). Conclusion: The proposed methods were simple, rapid, economic, accurate and precise; they were successfully applied for the determination of flucloxacillin-sodium in pure form and in pharmaceutical preparations.

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