Attia, K., Abdel-Aziz, O., Nasef, N., Mohamed, G. (2018). Determination of Flucloxacillin-sodium in Binary Mixture with Ampicillin-trihydrate Using Univariate and Multivariate Spectrophotometric Methods: A Comparative Study. Journal of Advanced Pharmacy Research, 2(3), 180-190. doi: 10.21608/aprh.2018.2362.1047
Khalid Abdel-Salam Attia; Omar Abdel-Aziz; Nancy Nasef; Ghada Mohamed. "Determination of Flucloxacillin-sodium in Binary Mixture with Ampicillin-trihydrate Using Univariate and Multivariate Spectrophotometric Methods: A Comparative Study". Journal of Advanced Pharmacy Research, 2, 3, 2018, 180-190. doi: 10.21608/aprh.2018.2362.1047
Attia, K., Abdel-Aziz, O., Nasef, N., Mohamed, G. (2018). 'Determination of Flucloxacillin-sodium in Binary Mixture with Ampicillin-trihydrate Using Univariate and Multivariate Spectrophotometric Methods: A Comparative Study', Journal of Advanced Pharmacy Research, 2(3), pp. 180-190. doi: 10.21608/aprh.2018.2362.1047
Attia, K., Abdel-Aziz, O., Nasef, N., Mohamed, G. Determination of Flucloxacillin-sodium in Binary Mixture with Ampicillin-trihydrate Using Univariate and Multivariate Spectrophotometric Methods: A Comparative Study. Journal of Advanced Pharmacy Research, 2018; 2(3): 180-190. doi: 10.21608/aprh.2018.2362.1047
Determination of Flucloxacillin-sodium in Binary Mixture with Ampicillin-trihydrate Using Univariate and Multivariate Spectrophotometric Methods: A Comparative Study
1Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, Egypt.
2Pharmaceutical Analytical Chemistry, Faculty of pharmacy, Ain shams Univeristy
Abstract
Objectives: The aim of this study was to develop four simple and accurate spectrophotometric methods for determination of flucloxacillin-sodium in binary mixture with ampicillin-trihydrate without separation. Methods: One of them was a univariate constant center method and the other three methods were multivariate chemometric methods named; Savitsky-Golay filters, continuous wavelet transform of ratio spectra and wavelet transform of first derivative of the ratio spectra. Results: The proposed methods adopted for selective determination of flucloxacillin-sodium and obey Beer’s law in the range (2-20 µg mL-1). Conclusion: The proposed methods were simple, rapid, economic, accurate and precise; they were successfully applied for the determination of flucloxacillin-sodium in pure form and in pharmaceutical preparations.
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