Essa, A., Mohamed, T., Haggag, E., Elkhrisy, E. (2018). Flavonolignans and Biological Activity of Nannorrhops ritchiana Leaves. Journal of Advanced Pharmacy Research, 2(1), 44-55. doi: 10.21608/aprh.2017.4721
Ahmed F. Essa; Tahia Mohamed; Eman Haggag; Ezz EL-Din Elkhrisy. "Flavonolignans and Biological Activity of Nannorrhops ritchiana Leaves". Journal of Advanced Pharmacy Research, 2, 1, 2018, 44-55. doi: 10.21608/aprh.2017.4721
Essa, A., Mohamed, T., Haggag, E., Elkhrisy, E. (2018). 'Flavonolignans and Biological Activity of Nannorrhops ritchiana Leaves', Journal of Advanced Pharmacy Research, 2(1), pp. 44-55. doi: 10.21608/aprh.2017.4721
Essa, A., Mohamed, T., Haggag, E., Elkhrisy, E. Flavonolignans and Biological Activity of Nannorrhops ritchiana Leaves. Journal of Advanced Pharmacy Research, 2018; 2(1): 44-55. doi: 10.21608/aprh.2017.4721
Flavonolignans and Biological Activity of Nannorrhops ritchiana Leaves
1Department of Chemistry of Natural Compounds, National Research Centre, El Bohouth St., Dokki, Giza, 12622, Egypt.
2Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Ain Helwan, Cairo11795, Egypt
Abstract
Objectives: This study aimed at isolating the polyphenolic compounds of 70% methanol extract ofNannorrhops ritchiana Griff. leaves and assaying the antioxidant and cytotoxic activities of the extract and main fractions. Methods: The methanol extract of the leaves of N. ritchiana was chromatographically fractionated using a bioactivity-guided approach. The isolated compounds were spectroscopically elucidated by UV, MS, 1H/13C NMR and 2D NMR spectroscopic techniques. The radical scavenging activity of the methanol extract and its fractions was evaluated using DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging assay and their cytotoxic activity was assayed using SRB (3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide) test against human hepatocellular liver carcinoma (HepG2), human alveolar adenocarcinoma (A549) and human prostate carcinoma (PC3) cell lines. Results: Eight compounds were isolated and identified for the first time from the leaves of N. ritchiana, comprising five flavonolignans;Indocalatin A (4) which is a rare compound that has been reported only once in nature, 9″-O-glucopyranosyl Salcolin A (5), 9″-O-glucopyranosyl Salcolin B (6) together with their aglycones, Salcolin A (7) and Salcolin B(8) along with three flavone glycosides; Tricin-7-O-rutinoside (1), Orientin (2) and Isoorientin (3). The methanol extract of N. ritchiana showed antioxidant activity with SC50 39.4 ± 1.06 μg/ml, while fractionIIshowed significant effect with SC50 of 6.1 ± 0.24 μg/ml in comparison with ascorbic acid (SC50 1.8 ± 0.35 μg/ml). The methanol extract and the fraction III showed a promising cytotoxic activity against selected cell lines especially the A549 with IC50 9.5 ± 1.98 μg/ml and HepG2 with IC50 8.15 ± 1.3 μg/ml, respectively.Conclusion:N. ritchiana leaf methanol extract is a new source of biologically active compounds, including flavonolignans.
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